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Benchmark Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various
sizes (150 bp to 6 kbp).
It’s low EEO (≤ 0.13-m ) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.
Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols.
It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H O), and exhibits exceptionally low absorption of
chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.
Features: |
| Moisture: |
≤ 7% |
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| EEO (electroendosmosis): |
≤ 0.13 –mr |
| Sulfate: |
≤ 0.20% |
| Gelling Temp. (at 1.5%): |
36° ± 1.5°C |
| Melting Temp. (at 1.5%): |
88° ± 1.5°C |
| Gel Strength (at 1.0%): |
1200g/cm² |
| Gel Strength (at 1.5%): |
2500g/cm² |
| DNase / RNase: |
None Detected |
| Protease: |
None Detected |
| Endonuclease: |
None Detected |
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| Ordering Information : |
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| A1700 |
Agarose LE, Organic Solvent Free, 25g |
| A1701 |
Agarose LE, Organic Solvent Free, 100g |
| A1705 |
Agarose LE, Organic Solvent Free, 500g |
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