Implen LabelGuard Microliter Cell
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The innovation for UV/Vis based nucleic acid and protein analysis
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| With its innovative optical pathway the cell is designed for optimum measurement results with low sample volumes starting from 0.7 µl. The sample is pipetted onto the centre of the measuring window. Due to the integrated beam deflexion and the use of fibre-optic light conductors the sample can be measured directly on the measurement window. Using one of the two available lids of the microliter cell (lid 10, lid 50) different liquid columns of defined pathlength are created. Virtual dilutions of up to 1/50 are generated in comparison to a standard 1 cm cuvette measurement, without a manual dilution of the sample. Dilution errors are avoided and the sample can be retrieved after the measurement for further processing. The LabelGuard™ Cell is compatible in most commercially available UV/Vis spectrophotometers. |
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Features:
- Spectrophotometrical analysis of submicroliter nucleic acid and protein samples.
Required sample volume: 0.7 µl - 5 µl
- Excellent reproducibility and a simple handling procedure
Retrieval of sample, fast to clean, no cross contaminations
- No dilution errors due to a virtual dilution of up to 1:100
Dynamic range for ds-DNA: 2 ng/µl - 5.000 ng/µl
- Compatible with most commercial UV/Vis spectrophotometers
Wavelength range: 190 nm - 1100 nm
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| The LabelGuard™ Microliter Cell is designed for the following applications: |
• Nucleic Acid Analysis
Nucleic Acids Quantification
Nucleic Acids Estimation of Purity
Dye incorporation rate
• Analysis of samples in organic compounds
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• Protein Analysis
A280
A280 Dye incorporation
• All UV/Vis analysis utilizing the wavelength range of 190 to 1100 nm |
Average detection range and required volumes for undiluted Nucleic Acid Samples |
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substance specific factor |
Lid 10 Dilution factor 10 Pathlength 1 mm [ng/µl] |
Lid 50 Dilution factor 50 Pathlength 0.2 mm [ng/µl] |
Total Detection Range [ng/µl] |
| dsDNA |
50 |
25 - 600 |
125 – 3.000 |
25 – 3.000 |
| ssDNA |
37 |
19 - 444 |
93 – 2.220 |
19 – 2.220 |
| RNA |
40 |
20 - 480 |
100 – 2.400 |
20 – 2.400 |
| Oligo |
33 |
17 - 396 |
83 – 1.980 |
17 – 1.980 |
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| Corresponding Absorbance Value Limits for A 260 |
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0,05 to 1,2 Abs |
0,05 to 1,2 Abs |
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| Required Sample Volume |
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3 to 5 µl |
0.7 to 4 µl |
| The detection range can be even enlarged with the NanoPhotometer™ (e.g. dsDNA 2- 8.000 ng/µl) |
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Operating the LabelGuard™ Microliter Cell is as simple as it could be:
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Step 1
Insert the LabelGuard™ Microliter Cell into the cellholder with the cell windows facing the light beam |
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Step 2
The sample (0.7 to 5 µl) is pipetted directly onto the active measurement window |
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Step 3
Closing of the lid. |
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Step 4
Taking the measurement. After the measurement the sample can be retrieved and stored for later use. |
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Step 5
Cleaning of the measurement window is easily performed with a fluff free tip or Kimwipe – no cross contamination, no dilution errors, no carry over effects |
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Step 6
Removing of sample residues from the lid mirror with a fluff free tip or Kimwipe. The photometer is ready for the next sample. |
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Technical Specifications:
| Wavelength Range: |
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190-1100 nm |
| Operating Temperature: |
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5 to 50°C |
| Dimensions |
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12.5 x 12.5 x 69.5 mm (8.5 mm centre height)
12.5 x 12.5 x 76.0 mm (15 mm centre height)
12.5 x 12.5 x 81 mm (20 mm centre height) |
| Sample Recovery |
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yes (no evaporation) |
| Cleaning Time |
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5 sec |
Compatible with volatile solvent
(e.g. acetonitrile/toluene) |
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yes |
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